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Probing the interactions of ZEN, Z14S, and Z14GA with CYP1A2 (A), CYP2C9 (B), and CYP3A4 (C) enzymes. Effects of positive control inhibitors (magenta; α-NF, α-naphthoflavone; SULF, <t>sulfaphenazole;</t> KET, ketoconazole), ZEN (blue), Z14S (red), and Z14GA (green) (each 20 μM). Data represent means ± SD ( n = 3; ** p < 0.01), all samples contained uniformly 0.2 v/v% DMSO.
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Probing the interactions of ZEN, Z14S, and Z14GA with CYP1A2 (A), CYP2C9 (B), and CYP3A4 (C) enzymes. Effects of positive control inhibitors (magenta; α-NF, α-naphthoflavone; SULF, <t>sulfaphenazole;</t> KET, ketoconazole), ZEN (blue), Z14S (red), and Z14GA (green) (each 20 μM). Data represent means ± SD ( n = 3; ** p < 0.01), all samples contained uniformly 0.2 v/v% DMSO.
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sulfaphenazole  (MedChemExpress)
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Probing the interactions of ZEN, Z14S, and Z14GA with CYP1A2 (A), CYP2C9 (B), and CYP3A4 (C) enzymes. Effects of positive control inhibitors (magenta; α-NF, α-naphthoflavone; SULF, <t>sulfaphenazole;</t> KET, ketoconazole), ZEN (blue), Z14S (red), and Z14GA (green) (each 20 μM). Data represent means ± SD ( n = 3; ** p < 0.01), all samples contained uniformly 0.2 v/v% DMSO.
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Probing the interactions of ZEN, Z14S, and Z14GA with CYP1A2 (A), CYP2C9 (B), and CYP3A4 (C) enzymes. Effects of positive control inhibitors (magenta; α-NF, α-naphthoflavone; SULF, sulfaphenazole; KET, ketoconazole), ZEN (blue), Z14S (red), and Z14GA (green) (each 20 μM). Data represent means ± SD ( n = 3; ** p < 0.01), all samples contained uniformly 0.2 v/v% DMSO.

Journal: ACS Omega

Article Title: In Vitro Evaluation of the Potential Interactions of Zearalenone-14-sulfate and Zearalenone-14-glucuronide with Human Cytochrome P450 Enzymes, Organic Anion Transporting Polypeptides, and ATP-Binding Cassette Multidrug Transporters

doi: 10.1021/acsomega.5c05217

Figure Lengend Snippet: Probing the interactions of ZEN, Z14S, and Z14GA with CYP1A2 (A), CYP2C9 (B), and CYP3A4 (C) enzymes. Effects of positive control inhibitors (magenta; α-NF, α-naphthoflavone; SULF, sulfaphenazole; KET, ketoconazole), ZEN (blue), Z14S (red), and Z14GA (green) (each 20 μM). Data represent means ± SD ( n = 3; ** p < 0.01), all samples contained uniformly 0.2 v/v% DMSO.

Article Snippet: Diclofenac, 4′-hydroxydiclofenac, and sulfaphenazole were from Biosynth (Berkshire, U.K.).

Techniques: Positive Control